Services

We provide a variety of DNA cloning services to research labs worldwide. LBL scientists bring you high quality of service products by experienced handling at our laboratory.

Benefits with our services:

• Timesaving: Reduced turn around time to speed up your research development and increase the efficiency in your lab.
• High quality: DNA cloning products are sequenced to ensure quality and accuracy.
• Cost-effective: Our custom services are flexible and reliable with competitive prices to meet your financial needs without compromising your research capabilities.

CAD = Canadian dollar

To make a quotation, contact us online or send your request to us by e-mail at: info@lanleys.com

Bulk Discount: We offer significant discounts on bulk orders (> 10 service items) for research labs in pharmaceutical and biotech companies and research institutes. All bulk orders will be delivered within designated time whether in a single P.O. or over a period of time.

Privacy Policy: In LBL, each order is subject to stringent confidentiality regulations. Our clients' information has been well protected following our out-standing security measurements.

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DNA Cloning

We can clone various DNA fragments into a plasmid vector provided or authorized by you. Simply provide your starting materials, let us do the rest. Our DNA cloning services include but not limited to:

• Standard subcloning of a DNA insert fragment from one vector to another;
• N-terminal and C-terminal tag integration;
• Promoter-reporter cloning;
• Multiple cloning site changes;

Plasmid vector and DNA insert fragment will be prepared by restriction digestion or PCR with low cycle, high-fidelity amplification from a template. After ligation and transformation, clones will be screened for the correct restriction fragments and for orientation, if necessary. When PCR has been applied, product will be sequenced to ensure sequence integrity. Finally, you will receive a mini-scale plasmid DNA preparation and a detailed project report.

Large insert/vector: We have expertise to perform long (> 4 kb) DNA fragment ligation and DNA subcloning with large size (> 10 kb) vectors. By using our services, you will never become frustrated with difficult inserts and vectors anymore. Focus on your research and move on.

We deliver over 80% of all constructs within 1 to 3 weeks. Time may vary depending on the volume of order and the complexity of the cloning procedure. Customers will be informed in the Purchase Order Confirmation.

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De Novo PCR Cloning

If you are planning to work on a gene that you don't have, or if you want to have a genomic DNA fragment for study, we can clone a gene or gene fragment for you from genomic/cDNA libraries of human, mouse and rat species. Genes from other species may also be available on request.

Tell us the name or Genbank accession number of the gene that you are interested. We will design and supply the PCR primers to amplify the gene, and subcloned it into the plasmid vector of your choice.

All PCR products will be sequenced (both DNA strands) to ensure sequence integrity. Finally, you will receive a mini-scale plasmid DNA preparation and a detailed project report.

RT-PCR cloning: If you have a gene, a special EST or gene isoform that you know about the sequence, we will design and supply the PCR primers to amplify the target sequence from RNA templates. Simply provide us with DNA sequence information and tissue, cells or RNA samples, we will do the rest for you.

We deliver over 80% of all constructs within 1 to 3 weeks. Time may vary depending on the volume of order and the complexity of the cloning procedure. Customers will be informed in the Purchase Order Confirmation.

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Site-directed Mutagenesis

We can carry out site-directed mutagenesis on the selected sequences to make specified base changes including deletions, insertions and substitutions, into a gene of your interest.

All mutagenesis clones are sequenced to ensure that the desired base changes have been made and to ensure sequence integrity of the remainder of the clone. Simply send us your clone and a list of the mutations you want. You will receive a mini-scale plasmid DNA preparation and a detailed project report.

We deliver over 80% of all constructs within 1 to 3 weeks. Time may vary depending on the volume of order and the complexity of the cloning procedure. Customers will be informed in the Purchase Order Confirmation.

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