General cDNA Expression

 List of expression vectors and prices

RNAi experiments often require expression of cDNAs. Initially you may need to co-express your cDNA and the shRNA/miRNA to validate your RNAi target sequence. Later, expression of a heterogenic cDNA to rescue the phenotype of RNAi can be essential to provide a convincing result.

RNAi Combination Toolbox™ consists of sets of plasmid DNA vectors with the most flexibility that allows you to perform RNAi technology for various research purposes. Among these vectors, multiple cloning sites for cDNA subcloning are fully compatible (Table). Notably, most of them can be found either identical or compatible to those that are currently being used in your lab.

Table. Multiple cloning sites1 available for cDNA subcloning

Strand Multiple Cloning Sites + NheI2, PmeI, KpnI, Bsp1407I, BglII3, MluI, EcoRI, PstI, SmaI, HpaI, NotI, SacII, SpeI4 - NheI2, SacII, NotI, HpaI, SmaI, PstI, EcoRI, MluI, BglII3, Bsp1407I, KpnI, PmeI, SpeI4

Notes:

1. Not all sites are unique in some vectors. Check technical information of each vector for details.
2. NheI is compatible to XbaI and SpeI.
3. BglII is compatible to BamHI.
4. SpeI is compatible to XbaI and NheI.

The vectors carry traditional CMV promoter, which will give you robust expression in a wide range of mammalian cell lines.