In Vivo Expression of shRNA/miRNA

 List of expression vectors and prices

Studies have shown that shRNAs expressed from a plasmid vector in the cells with stem-loop structures can be processed by Dicer into siRNAs, and subsequently enter RISC slightly faster than conventional synthetic siRNA duplexes.

RNAi Combination Toolbox™ provides you various plasmid-based DNA vectors, which allow you to express shRNA/miRNA in the cells by conventional DNA transfection. Expressions of shRNA/miRNA are driven by human U6 promoter (Figure 1).

Figure 1

Notably, multiple cloning sites are identical among all these vectors. DNA hairpins can be moved from one vector to another by simple steps of DNA subcloning.

Such DNA subcloning can also be easily conducted between in vivo expression vectors and in vitro expression vectors (Figure 2).

Figure 2

The shRNA/miRNA hairpins made from in vitro expression vectors and in vivo expression vectors are slightly different, but functionally interchangeable as far as for gene silencing concerned.