Expression of Rescue cDNA

 List of expression vectors and prices

To validate the result from an RNAi experiment, expression of a cDNA that is insensitive to RNAi to rescue the phenotype is often required. If you are studying the structure-related gene function, expression of a gene mutant during the knockdown of its wild type counterpart might be found very helpful (Figure 1).

Figure 1

To ensure the co-expression of shRNA/miRNA and the cDNA of your interest, LBL scientists have developed novel plasmid vectors among RNAi Combination Toolbox™, which allow you to express shRNA/miRNA and cDNA from a single vector (Figure 2).

Figure 2

These vectors contain human U6 promoter-driven RNAi expression cassettes and CMV promoters followed by conventional cDNA multiple cloning sites. Some vectors may contain SV40 early promoter for cDNA expression with respect to your different research purposes.