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Frequently Asked QuestionsAntibody Products
ServicesWhat is the source of your antibody products? All antibodies are made in-house, and thus the quality of our antibody products and their long-term supplies are guaranteed. In LBL, we are trained and certified to handle animal experiments by following the guidelines set by The Canadian Council on Animal Care (CCAC). Animals are housed at the Center of Biological Experiments, which is located in the Laval campus of INRS/Universite d'avant-garde. What is cell culture supernatant? Cell culture supernatant refers to the cell culture media, which contains antibodies secreted from cultured hybridoma cells. This is a standard method recommended by CCAC and AAALAC for in vitro production of mouse monoclonal antibodies in large scale, and such prepared antibodies can be used for most applications. How much is the yield of antibody in cell culture supernatant? The antibody concentration in cell culture supernatant varies among hybridoma clones, and it may be up to 100 μg/ml on an average of 50 μg/ml (Shepherd & Dean, 2000). Can I freeze the antibody? Yes, antibody can be frozen and stored at -20°C for many years. However, freeze-and-thaw cycles will greatly affect the biological activities of antibodies. Therefore, make aliquotes before moving them into a freezer. Is it possible to use antibody on other species? Yes, it is possible. Cytoskeletal proteins such as actin and tubulin, are highly conserved among species. We validate our antibodies on mouse and human cells, which are most popularly used in medical research. Other species are not included in our tests as yet. Do you make polyclonal antibodies? We produce polyclonal antibodies from mouse, but not from other species. My project is confidential. How do you handle this work? We can sign a confidentiality agreement upon request. If you still have questions that are not found in the list, or if you want to know more about our products and services in general, send your questions to our Technical Support Team by e-mail at: support@lanleys.com |
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