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Immunofluorescence (IF): Fluorescent images of stably transfected HT-1080 cells in the absence or presence of SARS-CoV-2 spike protein, fixed and labelled with mouse monoclonal anti-spike antibody. Goat anti-Mouse IgG (H+L) conjugated with Alexa Fluor™ 568 was used as a secondary antibody (red). Nuclei were counterstained with Hoechst 33342 (blue). |
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Product Information
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Gene Symbol:
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SPIKE_SARS2
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Molecular Weight:
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141 kDa (full-length), but it appears as ~180 kDa in SDS-PAGE.
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Source:
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This is a mouse monoclonal antibody raised against the spike protein from SARS-CoV-2.
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Clone #:
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4B9
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Isotype:
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IgG1
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Specificity:
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This antibody detects the spike protein of SARS-CoV-2, but not that of SARS-CoV-1.
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Physical Form:
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Freeze-dried powder from 1 × PBS solution, or cell culture media with 0.02% NaN3
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Application:
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• Western blotting (WB): 1:1,000 dilution
• Immunoprecipitation (IP): 1:100 dilution
• Flow cytometry (Flow): 1:100 dilution
• Immunofluorescence (IF): 1:50 dilution
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Storage:
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Store freeze-dried powder at 2 - 8°C upon arrival. When ready to use, rehydrate with desired volume of distilled H2O and centrifuge if not clear. For long-term storage, make smaller aliquots and keep them at -20°C or below. Avoid repeated freezing and thawing cycles.
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Background
Coronaviruses are a family of enveloped positive-sense RNA viruses ranging from 60 nm to 140 nm in diameter with trimeric spike proteins on its surface, which gives it a crown-like appearance under electron microscope. Each spike protein can be cleaved at two sites: S1/S2 and S2'. S1 is responsible for binding to the host cell receptor and S2 is for the fusion of the viral and cellular membranes. Angiotensin converting enzyme 2 (ACE2) has been identified as the receptor for both SARS-CoV-1 and SARS-CoV-2, the latter causing coronavirus disease COVID-19. The receptor-binding domain (RBD) is a subdomain of S1, which interacts with the extracellular binding site on ACE2 known as the peptidase domain (PD). Such interaction is central for virus transmission and considered as primary antiviral drug target.
Important Note
This product is intended for research use only, not for use in human therapeutic or diagnostic procedures.
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